Miniaturized electrophoresis electrochemical protein sensor (MEEPS) for multiplexed protein detections
Published in Sensors and Actuators B: Chemical, 2017
We developed here a Miniaturized Electrophoresis Electrochemical Protein Sensor (MEEPS) platform that is capable of detecting specific and multiplexed signals from small volume of sample (10 μL) as well as reducing the effect of background interferences. The multiplexed capability and the reduced-effect of background interference are achieved using the electrophoresis unit of the MEEPS. The small foot-print system and sensitive signal detection are enabled via electrochemical sensors unit. Using cytokines as sample, results obtained from MEEPS reveal dynamic range of detection of representative cytokine, TNF-α, at 10 ng/mL–20 μg/mL and demonstrate multiplexed detections of four different cytokines IL-6, IFN-γ, TNF-α, and IL-8 from only 10 μL of sample.
Recommended citation: P. Kongsuphol, G. C. Lee, S. K. Arya, S. Y. Chiam, and M. K. Park, “Miniaturized electrophoresis electrochemical protein sensor (MEEPS) for multiplexed protein detections,” Sensors and Actuators B: Chemical, vol. 244, pp. 823–830, 2017.